Activity-Based Probes are small molecules (1-2 kDa) used in an enzyme activity investigation. They function as molecules that bind with an enzyme and allow for its detection. In order to achieve it, they are armed with three functional fragments: (1) a covalent warhead, (2) a specific sequence, and (3) a tag.
Current research on serine proteases was limited by the lack of specific tools for enzyme active form detection. Addressing this limitation, using HyCoSuL technology (Hybrid Combinatorial Substrate Library) established in our lab, we developed specific ABPs dedicated for all four neutrophil serine proteases, and we apply it in our research on neutropenias. This strategy allowed for better interaction of leading sequence within the enzyme active site. ABPs can be utilized in an early diagnosis and disease prevention, increasing the chances for recovery.